A putative new serotype of chicken infectious anemia virus (CIAV) isolated from 17-wk-old broiler breeder pullets was compared with a known, previously characterized CIAV isolate, the Del-Ros strain. Physicochemical characteristics evaluated included thermal stability, size, pH, and chloroform sensitivity. Physicochemically, CIAV-7 was identical to CIAV. The virus isolates were compared antigenically by enzyme-linked immunosorbent assay, virus neutralization, immunofluorescence assay, and western blot. All four serologic assays demonstrated that CIAV-7 is antigenically distinct from the Del-Ros strain of CIAV. Additionally, polymerase chain reaction (PCR) and Southern blot were used to determine if there were similarities in genome sequence between the two viruses. CIAV-7 could not be detected with CIAV-specific PCR primers or a with CIAV-specific probe by Southern hybridization.
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1 October 2002
Comparison of a Putative Second Serotype of Chicken Infectious Anemia Virus with a Prototypical Isolate II. Antigenic and Physicochemical Characteristics
E. Spackman,
S. S. Cloud,
J. K. Rosenberger
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Avian Diseases
Vol. 46 • No. 4
October 2002
Vol. 46 • No. 4
October 2002
antigenic characterization
chicken infectious anemia virus
physicochemical characterization
poultry